The screw positioning and fastening system is applied to the installation of automobile or train components. The screw position is located through the vision system, and the mechanical arm is fed back to tighten the screw. The system measures the tightening torque in real time during the tightening process to ensure that the screw tightening does reach the standard. The entire tightening system can automatically adapt to the pre-tightened screw angle and adjust the docking posture of the robotic arm.